Regulatory

Part:BBa_K2309001:Design

Designed by: Haohui Fang   Group: iGEM17_XJTLU-CHINA   (2017-09-25)


P2 promoter inducer precursor-AgrA (optimized for Lactococcus)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 451
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 451
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 451
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 451
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 451
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was adjusted to be Lactococcus expression-compatible


Source

The original coding sequence is from BBa_I746100 and is extracted from Staphylococcus aureus genomic DNA. Then it was optimized by our team for Lactococcus expression.

References